The Centre
Genetics equipment
© Antoni Costa
White room facility
Applications
Carefully controlled environments designed to perform sensitive procedures under sterile, contaminant-free conditions. These spaces are essential for techniques demanding precision and purity, such as the handling and amplification of environmental samples (eDNA and metabarcoding).
description
A carefully controlled environment where temperature, humidity, and the movement of materials and personnel are regulated to minimise the risk of contamination.
Key features
- Sterile surfaces: Constructed using non-porous materials designed for easy disinfection, effectively preventing the build-up of microorganisms.
- Zoning: Organized into distinct zones for preparation, handling, and storage, each area is subject to varying restrictions based on the required level of cleanliness.
- Use of specialized equipment: Equipment such as laminar flow cabinets, biosafety hoods, and automated tools ensure minimal environmental exposure.
Long-read Sequencer (Oxford Nanopore Promethion P2 SOLO)
Applications
Long-read sequencing of DNA and RNA fragments.
description
The PromethION P2 Solo is a third generation nanopore sequencer that enables the rapid sequencing of gigabases of data, without the need for amplification.
Key features
- Capacity: Accommodates up to two PromethION Flow Cells, which can operate independently or simultaneously.
- Read Lengths: Capable of sequencing reads ranging from 20 bases to over 4 million bases.
- Data Output: Each flow cell can generate up to 290 Gb of data, depending on factors like sample type and run conditions.
- Sample Multiplexing: Supports multiplexing of up to 96 samples per flow cell using PCR or PCR-free barcoding methods.
- Run Duration: Users have the flexibility to adjust the duration of sequencing runs to meet specific experimental requirements.
Short Fragment Sequencer (Illumina’s NextSeq1000)
Applications
Designed for massively parallel sequencing, the NextSeq 1000 enables whole-genome analysis, transcriptome profiling, and metagenomic studies.
description
The NextSeq 1000 is a synthesis sequencing system employing Illumina’s short-read technology, offering high accuracy and flexibility.
Key features
- High Capacity for Small and Medium-Sized Projects: Provides scalable output, making it suitable for various project sizes.
- Rapid Data Generation: Delivers high-quality data with reduced run times, enhancing laboratory efficiency.
- Seamless Bioinformatics Integration: Features onboard DRAGEN Bio-IT FPGA secondary analysis, simplifying data processing workflows.
- Versatile Application Compatibility: Supports a wide range of applications, including RNA-Seq, whole-genome sequencing (WGS), and epigenomics.
- Cost-Effective Sequencing: Offers reduced cost per base compared to other systems, making high-throughput sequencing more accessible..
Capillary Electrophoresis (Agilent 5200 Fragment Analyzer)
Applications
Designed for the quality control of nucleic acids, including genomic DNA (gDNA), messenger RNA (mRNA), next-generation sequencing (NGS) libraries, and polymerase chain reaction (PCR) products.
description
The Fragment Analyzer utilizes parallel capillary electrophoresis technology, enabling efficient and high-resolution analysis of nucleic acids.
Key features
- Sample Throughput: Analyses 12 samples per run (one for the ladder).
- Sample Volume: Requires only 2 µL per sample.
- Run Time: ~1 hour per run.
- Resolution: Up to 2 base pairs.
- RNA Quality Assessment: RQN score for total RNA integrity.
- Assay Compatibility: Supports NGS, large-fragment DNA, RNA, and other applications.
Biomark X9 System for High-Throughput Genomics
Applications
Designed for high-capacity quantification of DNA, RNA, and proteins, the Biomark X9 System supports gene expression studies, genotyping, epigenetics, and biomarker analysis across various fields, including molecular ecology, agrogenomics, pharmacogenomics, and pathogen detection.
description
The Biomark X9 System is an advanced microfluidics platform that enables high-resolution genomic analysis and multiplexing. It allows for 48×48 or 96×96 sample and assay configurations on a single integrated fluidic circuit (IFC) and supports subsequent separation for Illumina library preparation.
Key features
- High Capacity: Supports up to 9,216 nanoliter-scale reactions in a single run, ideal for large-scale studies.
- Microfluidic Technology: Reduces reagent consumption, optimizing costs and time.
- High Sensitivity: Accurately detects molecules at low concentrations, suitable for environmental DNA (eDNA) studies and limited samples.
- Advanced Software: Includes integrated tools for data analysis, graph generation, and management of large datasets.
- Automation: Reduces human error through automated sample preparation and analysis systems.
- Seamless Integration:Compatible with genomic databases and standard bioinformatics workflows.
Digital PCR System (Applied Biosystems QuantStudio Absolute Q)
Applications
Precise quantification of DNA and RNA molecules in gene expression studies, mutation detection, and gene copy number analysis.
description
The QuantStudio Absolute Q is a digital PCR (dPCR) system that partitions samples into thousands of individual reactions, enhancing sensitivity and accuracy.
Key features
- Absolute Quantification: Provides precise measurement of target nucleic acid molecules without the need for standard curves.
- High Sensitivity: Capable of detecting rare variants and low-abundance targets.
- Robust Performance: Minimizes interference from PCR inhibitors, ensuring reliable results even with challenging samples.
- Rapid Turnaround: Delivers results in as little as 90 minutes, increasing laboratory efficiency.
- User-Friendly Workflow: Integrates all dPCR steps into a single instrument with minimal hands-on time, simplifying the process.
Cryogenic mill (Retsch Cryomill)
Applications
Ideal for cryogenic grinding of biological samples, such as animal or plant tissues, using liquid nitrogen to maintain temperatures at -196°C.
description
The CryoMill is a laboratory ball mill specifically designed for cryogenic grinding. It features an integrated cooling system that continually cools the grinding jar with liquid nitrogen before and during the grinding process. This ensures that the sample is embrittled, facilitating the grinding of tough, elastic, or temperature-sensitive materials, and preserving volatile components.
Key features
- Sample Capacity: Accommodates 1 grinding jar per run, with jar volumes ranging from 5 ml to 50 ml.
- Vibration Frequency: Adjustable up to 30 Hz for efficient grinding.
- Grinding Modes: Supports cryogenic, dry, and wet grinding at ambient temperature.
Nucleic Acid Fragmenter (Diagenode MegaRuptor 3)
Applications
DNA fragmentation for library preparation in third-generation sequencing and structural genomics.
description
The MegaRuptor 3 is specialized equipment that generates DNA fragments of defined sizes, ideal for projects utilizing PacBio and Oxford Nanopore technologies.
Key features
- Fragmentation Range: Uniformly fragments DNA from 5 kb to over 100 kb.
- Throughput: Processes up to 8 samples simultaneously.
- Sample Volume: Accommodates volumes ranging from 65 µl to 500 µl.
- Compatibility: Effective with both high and low-concentration DNA samples, independent of source, temperature, or salt content.
Nucleic Acid Sonicator (Diagenode Bioruptor Pico)
applications
Ideal for cell disruption, DNA/RNA fragmentation, and sample sonication in library preparation.
description
The Bioruptor Pico is an ultrasonic system that utilizes low-intensity energy to precisely fragment nucleic acids.
key features
- Uniform Fragmentation: Achieves DNA shearing in the range of 150–1,000 bp.
- Sample Capacity: Processes up to 12 samples simultaneously using 0.65 ml tubes.
- Temperature Control: Features an integrated temperature control system to prevent sample degradation.
- Application Compatibility: Suitable for various applications, including ChIP-Seq and metagenomics.
DNA Size Selection System (BluePippin, by Sage Science)
Applications
Precise separation of DNA fragments by size, essential for library preparation in third-generation sequencing platforms such as PacBio and Oxford Nanopore.
description
The BluePippin is an automated system that employs pulsed-field electrophoresis to select DNA fragments within specific size ranges, enhancing the quality of sequencing libraries.
Key features
- Broad Size Range: Capable of selecting DNA fragments from 100 base pairs up to 50 kilobases, accommodating various sequencing requirements.
- High Resolution: Provides precise and reproducible size selection, improving downstream sequencing accuracy.
- User-Friendly Operation: Utilizes pre-cast, disposable agarose gel cassettes for straightforward setup and minimal hands-on time.
Pipetting Robot (ASSIST PLUS by INTEGRA)
applications
Automates pipetting protocols for high-throughput genomics experiments and library preparation.
description
The ASSIST PLUS is a robotic system that performs pipetting tasks with high precision, minimizing manual intervention and improving experimental reproducibility.
Key features
- Pipette Compatibility: Supports all INTEGRA electronic multichannel pipettes, including fixed and adjustable tip spacing models, as well as the D-ONE single-channel pipetting module.
- Intuitive User Interface: Easily configure and select programs directly from the pipette’s interface, enabling quick setup of custom protocols.
- Versatile Plate Compatibility: Works with a wide range of labware, from tubes to 384-well plates, in both horizontal and vertical orientations.
- Error Reduction: Automates repetitive pipetting tasks, minimizing human error and ensuring consistent experimental results.
PCR Workstation (UVC/T-AR by BioSan)
applications
Provides a controlled environment for PCR sample preparation, minimizing the risk of cross-contamination.
description
The UVC/T-AR is a PCR workstation equipped with UV-C lamps for decontamination, ensuring a sterile workspace during sample handling.
key features
- UV Decontamination: Features an open UV lamp that disinfects the working area by inactivating DNA/RNA fragments within 15–30 minutes of exposure.
- Controlled Ventilation: Includes a bactericidal flow-type recirculator providing permanent decontamination inside the workstation during operation.
- Ergonomic Design: Compact and bench-top design with metal framework and plexiglass walls, suitable for PCR and nucleic acid work.
Laminar Flow Cabinet (Alpina H120)
applications
Creates a contaminant-free environment for handling sensitive samples in genomics and microbiology.
description
The Alpina H120 is a cabinet with unidirectional laminar airflow that protects samples from external contaminants.
key features
- HEPA Filters: Ensures sterile air by filtering out particulates.
- Compact Design: Optimized for laboratory space with low energy consumption.
- Illumination: Internal LED lighting provides clear visibility of the workspace.
- Visibility: Transparent panels allow for optimal viewing during operations.
UV-C Decontamination Chamber (by Purion)
applications
Rapid sterilization of laboratory equipment and materials, including pipettes and molecular tools.
description
An enclosed chamber equipped with UV-C lamps designed to eliminate microorganisms and contaminants from laboratory instruments.
key features
- Dual UV-C Lamps: Enhances efficiency in decontamination processes.
- Capacity: Accommodates multiple objects simultaneously for sterilization.
- Adjustable Timer: Allows users to set sterilization durations according to specific needs.
Spectrophotometer (IMPLO NP80 Mobile)
applications
Quantification and purity assessment of nucleic acids and proteins in DNA, RNA, and protein samples.
description
The IMPLO NP80 Mobile is a spectrophotometer designed for fast and reliable measurements in the laboratory.
key features
- Concentration Detection: Offers a wide range for detecting various sample concentrations.
- User Interface: Features an intuitive touch interface with mobile connectivity for ease of use.
- Microvolume Capability: Requires minimal sample volumes without the need for additional reagents.
- High Precision: Utilizes direct light beam technology to ensure accurate measurements.
Fluorometer (Qubit Flex by Invitrogen)
applications
Accurate quantification of DNA, RNA, and proteins using fluorescent methods in molecular studies.
description
The Qubit Flex is designed to measure low concentrations of biomolecules using specific fluorescent probes.
key features
- High Sensitivity: Especially effective at detecting low DNA/RNA concentrations.
- Throughput: Capable of processing up to 8 samples simultaneously for increased efficiency.
- User-Friendly Interface: Equipped with a digital interface and connectivity to databases.
- Compatibility: Works seamlessly with Qubit assay kits for various types of analyses.
ThermoMixer C (by Eppendorf)
applications
Simultaneous incubation and mixing of samples to optimize molecular reactions such as PCR, enzymatic digestion, and library preparation.
description
The ThermoMixer C is a versatile device that combines mixing and temperature control functions in one unit.
key features
- Temperature Range: Operates between 4°C to 100°C, accommodating various experimental needs.
- Adaptability: Features interchangeable adapters compatible with different types of tubes and plates.
- Mixing Efficiency: Provides homogeneous mixing with adjustable speed settings.
- Digital Display: Offers an intuitive interface for programming and monitoring experiments.
Gel Imaging System (Bio-Rad GelDoc Go)
applications
Imaging electrophoresis gels for DNA, RNA, and protein analysis.
description
The GelDoc Go is a compact gel imaging system designed to document and analyze fluorescent or gel-stained bands.
key features
- Dye Compatibility: Supports both fluorescent and non-fluorescent dyes, including ethidium bromide (EtBr) and SYBR stains.
- User-Friendly Interface: Equipped with Image Lab Touch Software for intuitive operation and analysis.
- High-Quality Imaging: Captures high-resolution, publication-quality images of nucleic acid and protein
- Compact Design: Features a small footprint, ideal for laboratories with limited space.
Thermal Cyclers 96/384 Well (Biometra TAdvanced by Analytik Jena)
applications
PCR amplification and incubation.
description
The Biometra TAdvanced is a thermal cycler designed for precise PCR amplification, accommodating both 96- and 384-well sample plates.
Key features
- Versatile Block Options: Features aluminum blocks compatible with 0.2 ml tubes, 8-tube strips, and 96- or 384-sample plates.
- Wide Temperature Range: Operates from 4°C to 99.9°C, suitable for various PCR protocols.
- Gradient Function: Offers a gradient range between 20°C to 99°C for assay optimization.
- High Accuracy: Ensures temperature precision with an accuracy of ±0.1°C.
Refrigerated Centrifuges (Eppendorf 5418 R & 5430 R)
applications
Centrifugation at controlled temperatures for various sample types.
description
Eppendorf’s 5418 R and 5430 R are compact refrigerated centrifuges designed for efficient sample processing in molecular biology applications.
key features
- Capacity: Rotors for up to 48 tubes of 1.5/2ml conical bottom
- Versatile Rotor Options: Supports various rotors, including those for Eppendorf tubes, PCR strips, microplates, and 15/50 ml conical tubes.
- High Speed: Reaches up to 30,130 × g (17,500 rpm) for diverse applications.
- Temperature Range: Offers a temperature control range from -11°C to 40°C for sensitive samples.